Semisynthesis of a Homogeneous Glycoprotein Enzyme: Ribonuclease C: Part 1 (pages 1936–1940)
Christian Piontek, Petra Ring, Olaf Harjes, Christian Heinlein, Stefano Mezzato, Nelson Lombana, Claudia Pöhner, Markus Püttner, Daniel Varón Silva, Andreas Martin, Franz Xaver Schmid and Carlo Unverzagt
Version of Record online: 28 JAN 2009 | DOI: 10.1002/anie.200804734
Seven in one blow: The efficient formation of mixed disulfides on the thiol-rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins.