A Protease-Based Strategy for the Controlled Release of Therapeutic Peptides (pages 4930–4933)
Hongjian Li, Yi Ma, Ying Chen, Yanxia Sang, Tianhong Zhou, Meilan Qiu, Xiumei Huang, Cindy Zhou and Zhengding Su
Article first published online: 10 JUN 2010 | DOI: 10.1002/anie.201000287
A polypeptide containing an albumin-binding domain (ABD), an amino acid sequence linker, and glucagon-like peptide-1 (GLP-1), a potential antidiabetic agent, is designed to slowly release GLP-1 through sequential cleavage by native plasma proteases (see picture). The half-life of the polypeptide in the bloodstream is extended by its association with human serum albumin (HSA).