Enzymatic Site-Specific Functionalization of Protein Methyltransferase Substrates with Alkynes for Click Labeling (pages 5170–5173)
Wibke Peters, Sophie Willnow, Mike Duisken, Henning Kleine, Thomas Macherey, Kelly E. Duncan, David W. Litchfield, Bernhard Lüscher and Elmar Weinhold
Version of Record online: 22 JUN 2010 | DOI: 10.1002/anie.201001240
Pass and click: Protein methylation is an important posttranslational modification. Because the methyl group is a poor reporter group, new methods are needed to analyze methyltransferase substrates. A S-adenosyl-L-methionine-based cofactor was synthesized and used for the site-specific functionalization of proteins with alkynes by methyltransferases (first step) and subsequent labeling through CuAAC click chemistry (second step; see scheme).