Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering (pages 6111–6115)
Zbynek Prokop, Yukari Sato, Jan Brezovsky, Tomas Mozga, Radka Chaloupkova, Tana Koudelakova, Petr Jerabek, Veronika Stepankova, Ryo Natsume, Jan G. E. van Leeuwen, Dick B. Janssen, Jan Florian, Yuji Nagata, Toshiya Senda and Jiri Damborsky
Version of Record online: 19 JUL 2010 | DOI: 10.1002/anie.201001753
In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β-bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for α-bromoesters.