A Straightforward Approach for Cellular-Uptake Quantification (pages 8240–8243)
Dr. David Paramelle, Dr. Gilles Subra, Lubomir L. Vezenkov, Dr. Marie Maynadier, Christophe André, Prof. Christine Enjalbal, Dr. Monique Calmès, Dr. Marcel Garcia, Prof. Jean Martinez and Dr. Muriel Amblard
Version of Record online: 23 SEP 2010 | DOI: 10.1002/anie.201003347
Putting a number on it: MALDI-TOF MS enabled direct quantification of the cellular uptake of cell-penetrating peptides (CPPs) by MDA-MB-231 breast cancer cells. This sensitive general strategy (see schematic representation), which requires no purification or separation steps, relies on the enhancement and discrimination of the MS signals of an α-cyano-4-hydroxycinnamic acid tag in a neutral α-cyano-4-hydroxycinnamic methyl ester matrix.