Chemical Evolution of a Bacterium’s Genome (pages 7109–7114)
Dr. Philippe Marlière, Julien Patrouix, Dr. Volker Döring, Prof. Dr. Piet Herdewijn, Sabine Tricot, Dr. Stéphane Cruveiller, Dr. Madeleine Bouzon and Prof. Dr. Rupert Mutzel
Version of Record online: 27 JUN 2011 | DOI: 10.1002/anie.201100535
Automated selection was used to evolve an Escherichia coli strain unable to synthesize thymine nucleotides into a chemically modified organism whose DNA genome is composed of adenine, cytosine, guanine, and an artificial base, the thymine analogue 5-chlorouracil. Evolving cells were initially observed as irregular filaments and progressively recovered the appearance of short rods typical of wild-type E. coli (see picture).