Enzymatically Incorporated Genomic Tags for Optical Mapping of DNA-Binding Proteins (pages 3578–3581)
Soohong Kim, Anna Gottfried, Ron R. Lin, Dr. Thomas Dertinger, Andrew S. Kim, Sangyoon Chung, Dr. Ryan A. Colyer, Prof. Dr. Elmar Weinhold, Prof. Shimon Weiss and Dr. Yuval Ebenstein
Version of Record online: 16 FEB 2012 | DOI: 10.1002/anie.201107714
All smiles: Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA) creates a fluorescence pattern of the T7 bacteriophage genome (see picture). The pattern is visualized as a linear optical barcode showing the genomic location of individual RNA polymerases bound to the DNA. The precision of the measurement presents new opportunities for contextual genomic research on the single-molecule level.