Polymer Nanofiber-Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells (pages 3379–3383)
Dr. Shuang Hou, Dr. Libo Zhao, Qinglin Shen, Dr. Juehua Yu, Charles Ng, Xiangju Kong, Dr. Dongxia Wu, Dr. Min Song, Dr. Xiaohong Shi, Xiaochun Xu, Wei-Han OuYang, Rongxian He, Prof. Xing-Zhong Zhao, Tom Lee, Prof. F. Charles Brunicardi, Dr. Mitch André Garcia, Prof. Antoni Ribas, Prof. Roger S. Lo and Prof. Hsian-Rong Tseng
Article first published online: 21 FEB 2013 | DOI: 10.1002/anie.201208452
Confined to one cell: A method to detect and isolate single circulating melanoma cells (CMCs; see figure) has been produced by integrating a polymer-nanofiber-embedded nanovelcro cell-affinity assay with a laser microdissection (LMD) technique. This method is able to separate CMCs from normal white blood cells (WBCs) and sequence individual cells for a specific mutation related to cancer progression, allowing for more personalized cancer therapy.