Enzymatic Conversion of Flavonoids using Bacterial Chalcone Isomerase and Enoate Reductase (pages 1439–1442)
Dipl.-Biol. Mechthild Gall, Dipl.-Biochem. Maren Thomsen, Dipl.-Biochem. Christin Peters, Dr. Ioannis V. Pavlidis, M. Sc. Patrick Jonczyk, M. Sc. Philipp P. Grünert, Dr. Sascha Beutel, Prof. Dr. Thomas Scheper, Egon Gross, Dr. Michael Backes, Dr. Torsten Geißler, Dr. Jakob P. Ley, Dr. Jens-Michael Hilmer, Dr. Gerhard Krammer, Dr. Gottfried J. Palm, Prof. Dr. Winfried Hinrichs and Prof. Dr. Uwe T. Bornscheuer
Version of Record online: 20 DEC 2013 | DOI: 10.1002/anie.201306952
The biocatalytic metabolic pathway for the degradation of flavonoids of Eubacterium ramulus was identified. A chalcone isomerase and an enoate reductase were successfully cloned, subsequently expressed in E. coli, and used under aerobic conditions, although E. ramulus is a strictly anaerobic bacterium. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones.