Structure of the Complex between a Heparan Sulfate Octasaccharide and Mycobacterial Heparin-Binding Hemagglutinin (pages 4192–4196)
Dr. Teng-Yi Huang, Dr. Deli Irene, Dr. Medel Manuel L. Zulueta, Tzu-Jui Tai, Shih-Han Lain, Dr. Cheng-Po Cheng, Dr. Ping-Xi Tsai, Dr. Shu-Yi Lin, Dr. Zhi-Geng Chen, Chiao-Chu Ku, Prof. Dr. Chwan-Deng Hsiao, Prof. Dr. Chia-Lin Chyan and Prof. Dr. Shang-Cheng Hung
Version of Record online: 15 MAR 2017 | DOI: 10.1002/anie.201612518
Clearly labeled: The structure of the complex between heparan sulfate and the mycobacterial heparin-binding hemagglutinin was characterized by NMR spectroscopy using uniformly 13C- and 15N-labeled forms of the sugar and protein. Notable chemical-shift perturbations occurred at the C-terminal residues of the protein. Molecular dynamics simulations based on the NMR data show electrostatic and hydrophobic interactions between the binding partners.