This paper is based on work presented at the 12th Symposium of the Japanese Arsenic Scientists' Society (JASS) held 5–6 November 2005 in Takizawa, Iwate Prefecture, Japan.
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Materials, Nanoscience and Catalysis
Isolation of monomethylarsonic acid-mineralizing bacteria from arsenic contaminated soils of Ohkunoshima Island†
Article first published online: 17 AUG 2006
DOI: 10.1002/aoc.1075
Copyright © 2006 John Wiley & Sons, Ltd.
Issue
1099-0739/asset/cover.gif?v=1&s=04bd9e08c0ca65d49547efbdbe335825939dd0de "Applied Organometallic Chemistry")
Applied Organometallic Chemistry
Special Issue: Special issue including information from the 12th Symposium of the Japanese Arsenic Scientists' Society (JASS) held 5–6 November 2005 in Takizawa, Iwate Prefecture, Japan
Volume 20, Issue 9, pages 538–544, September 2006
Additional Information
How to Cite
Maki, T., Takeda, N., Hasegawa, H. and Ueda, K. (2006), Isolation of monomethylarsonic acid-mineralizing bacteria from arsenic contaminated soils of Ohkunoshima Island. Appl. Organometal. Chem., 20: 538–544. doi: 10.1002/aoc.1075
- †
Publication History
- Issue published online: 17 AUG 2006
- Article first published online: 17 AUG 2006
- Manuscript Accepted: 18 JAN 2006
- Manuscript Received: 11 OCT 2005
Funded by
- Grant-in-aid for Encouragement of Young Scientists, Ministry of Education, Science, Sports and Culture. Grant Number: 14780441.
- Salt Science Research Foundation. Grant Number: 0424.
- Nissan Science Foundation.
- Abstract
- References
- Cited By
Keywords:
- organoarsenic;
- monomethylarsonic acid;
- MMAA mineralization;
- bacteria;
- arsenic contaminated soil
Abstract
Chemical warfare agents, composed of harmful organoarsenic compounds have contaminated the soils of Ohkunoshima Island with high levels of arsenic. As a basic research establishing useful bioremediation techniques, environmental factors such as arsenic concentrations and bacterial biomass in the soils were investigated. Among the five stations of Ohkunoshima Island, the soils of four stations were contaminated by high levels of arsenic compounds at concentrations of 125, 12.7, 3.29 and 0.504 g/kg soil, while the other station with low arsenic concentrations of 0.007 g/kg soil was considered an uncontaminated area. The distribution of arsenic compounds originating from the chemical weapon agent differs among the various areas of Ohkunoshima Island. The cell densities of arsenate-resistant bacteria also varied among the five stations, ranging from 106 to 108 cells/g soil. In an attempt to isolate bacteria that strongly mineralize the organoarsenic compounds, the mineralization activities for monomethylarsonic acid [MMAA(V)] of 48 isolates of arsenate-resistant bacteria were determined. Only nine isolates reduced 140 µg/l of MMAA(V), giving decreasing percentages ranging from 5 to 100% within 14 days. Among the nine isolates, two remarkably converted 140 µg/l of MMAA to more than 71 µg/l of inorganic arsenic. Presumably only specific members of the environmental bacterial population have strong mineralization activities for MMAA. Phylogenetic analysis using 16S rDNA sequences showed that the two isolates belonged to the Pseudomonas putida strains, which are known to have strong mineralization activity for various organic compounds. In the soil contaminated by arsenic at a high level, few bacteria in the arsenate-resistant bacterial group would significantly mineralize organoarsenic compounds. Copyright © 2006 John Wiley & Sons, Ltd.