Environment, Biology and Toxicology

Determination of degradation compounds derived from Lewisite by high performance liquid chromatography/inductively coupled plasma-mass spectrometry†

  1. Kenji Kinoshita1,*,
  2. Ozamu Shikino2,
  3. Yasuo Seto3,
  4. Toshikazu Kaise1

Article first published online: 17 AUG 2006

DOI: 10.1002/aoc.1104

Issue

Applied Organometallic Chemistry

Applied Organometallic Chemistry

Special Issue: Special issue including information from the 12th Symposium of the Japanese Arsenic Scientists' Society (JASS) held 5–6 November 2005 in Takizawa, Iwate Prefecture, Japan

Volume 20, Issue 9, pages 591–596, September 2006

Additional Information

How to Cite

Kinoshita, K., Shikino, O., Seto, Y. and Kaise, T. (2006), Determination of degradation compounds derived from Lewisite by high performance liquid chromatography/inductively coupled plasma-mass spectrometry. Appl. Organometal. Chem., 20: 591–596. doi: 10.1002/aoc.1104

Author Information

  1. 1

    Laboratory of Environmental Chemodynamics, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan

  2. 2

    PerkinElmer Japan Co. Ltd, 2-8-4 Kitasaiwai, Yokohama 220-0004, Japan

  3. 3

    National Research Institute of Police Science, Japan, 6-3-1 Kashiwanoha Chiba 277-0882, Japan

*Laboratory of Environmental Chemodynamics, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

  1. †

    This paper is based on work presented at the 12th Symposium of the Japanese Arsenic Scientists' Society (JASS) held 5–6 November 2005 in Takizawa, Iwate Prefecture, Japan.

Publication History

  1. Issue published online: 17 AUG 2006
  2. Article first published online: 17 AUG 2006
  3. Manuscript Accepted: 29 JUN 2006
  4. Manuscript Received: 10 MAY 2006

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Keywords:

  • chemical warfare agents;
  • Lewisite;
  • 2-chlorovinylarsenous acid (CVAA);
  • 2-chlorovinylarsonic acid (CVAOA);
  • HPLC/ICP-MS;
  • speciation analysis

Abstract

A method for determination of 2-chlorovinylarsenous acid (CVAA) and 2-chlorovinylarsonic acid (CVAOA), which are degradation compounds of the chemical warfare agent Lewisite, was examined by high-performance liquid chromatography/inductively coupled plasma-mass spectrometry (HPLC/ICP-MS). Inertsil C8 was suitable as the column and the mobile phase was consisted of 0.1% formic acid-acetonitrile (80:20). These compounds were detected sensitively in a short time and separated from inorganic arsenicals and diphenylarsinic acid (DPAA) and phenylarsonic acid (PAA), which are degradation compounds of diphenylchloroarsine and phenyldichloroarsine, respectively. The detection limits of CVAA and CVAOA were 0.2 and 0.1 ngAs/ml, respectively. In addition, a dynamic reaction cell and oxygen as the reaction gas were applied, and then arsenic was detected as AsO+ (m/z 91) in order to prevent interference by ArCl+ (m/z 75). This method was applied to the analysis of urine obtained from a CVAA-administered mouse and CVAOA was detected as the main metabolite. Thus, the speciation analysis of arsenic compounds derived from chemical warfare agents was achieved by HPLC/ICP-MS. Copyright © 2006 John Wiley & Sons, Ltd.

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