Research Article

Estimation of arsenobetaine in the NIES candidate certified reference material no. 18 human urine by HPLC–ICP-MS using different chromatographic conditions†

  1. Amit Chatterjee*,
  2. Yasuyuki Shibata,
  3. Jun Yoshinaga,
  4. Masatoshi Morita

Article first published online: 23 MAR 2001

DOI: 10.1002/aoc.142

Issue

Applied Organometallic Chemistry

Applied Organometallic Chemistry

Special Issue: JASS-9 Ninth Symposium of the Japanese Arsenic Scientists' Society

Volume 15, Issue 4, pages 306–314, April 2001

Additional Information

How to Cite

Chatterjee, A., Shibata, Y., Yoshinaga, J. and Morita, M. (2001), Estimation of arsenobetaine in the NIES candidate certified reference material no. 18 human urine by HPLC–ICP-MS using different chromatographic conditions. Applied Organometallic Chemistry, 15: 306–314. doi: 10.1002/aoc.142

Author Information

  1. National Institute for Environmental Studies, Environmental Chemodynamics Section, Environmental Chemistry Division, 16-2 Onogawa, Tsukuba, Ibaraki-3050053, Japan

*National Institute for Environmental Studies, Environmental Chemodynamics Section, Environmental Chemistry Division, 16-2 Onogawa, Tsukuba, Ibaraki-3050053, Japan

  1. †

    Based on work presented at the Ninth Symposium of the Japanese Arsenic Scientists' Society (JASS-9), held 21–22 November 1999 at Hiroshima, Japan.

Publication History

  1. Issue published online: 23 MAR 2001
  2. Article first published online: 23 MAR 2001
  3. Manuscript Accepted: 3 OCT 2000
  4. Manuscript Received: 9 DEC 1999

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Keywords:

  • arsenobetaine;
  • HPLC–ICP-MS;
  • LC-SCX;
  • Candidate Reference Material;
  • urine;
  • arsenic;
  • arsenic compounds

Abstract

HPLC–ICP-MS, employing a silica-based LC-SCX cation-exchange column, styrene–divinylbenzene copolymer-based PRP-X100 anion exchange column, an ODS reversed-phase and gel-permeation (polyvinyl alcohol-based resin) GS-220 columns, has been used for the separation, identification, and quantification of arsenic compounds, particularly arsenobetaine (AB), present in NIES candidate certified reference material (CRM) no. 18 human urine. AB is the predominant arsenic species, followed by dimethylarsinic acid, methylarsonic acid and arsenic acid. The peak of each arsenic compound has been validated by spiking of the authentic standard solution to the urine sample and by using the above chromatographic systems. The high concentration of chloride that co-elutes with the arsenic acid from the LC-SCX and with the AB from the GS-220 columns has interfered with the ion signals of arsenic acid and AB, by forming the molecular ions 40Ar35Cl+ and 38Ar37Cl+ in the plasma. Thus, the concentration of AB has been carefully estimated on the ­GS-220 after extracting the chloride interference (37Cl:35Cl = 1:3.1271) by measuring the 40Ar37Cl+. The peak of AB overlapped with the peak of arsenous acid and hindered the estimation of AB on the ODS and PRP-X100 columns. But AB has been baseline separated from the other arsenic compounds and also from the chloride with 20 mM pyridine at pH 2.60 on the LC-SCX. So, the LC-SCX column has been proven and used for the determination of AB in NIES candidate CRM no. 18 human urine. The concentrations of AB, estimated by the standard addition method and found using the LC-SCX and GS-220 columns, are 70.5 ± 5.5 (n = 20) and 71.5 ± 4 µgl−1 (n = 9). The concentration of AB thus found has been applied as the baseline value for the collaborative study to certify the AB in the NIES candidate CRM no. 18 human urine. Copyright © 2001 John Wiley & Sons, Ltd.

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