Deuterium isotope effect on the refractive index of liquids and its application to permeation chromatography
Article first published online: 9 MAR 2003
Copyright © 1971 John Wiley & Sons, Inc.
Journal of Applied Polymer Science
Volume 15, Issue 6, pages 1521–1525, June 1971
How to Cite
Carignan, Y. P. and Turngren, E. V. (1971), Deuterium isotope effect on the refractive index of liquids and its application to permeation chromatography. J. Appl. Polym. Sci., 15: 1521–1525. doi: 10.1002/app.1971.070150619
- Issue published online: 9 MAR 2003
- Article first published online: 9 MAR 2003
- Manuscript Revised: 10 MAR 1971
- Manuscript Received: 30 NOV 1970
Differences in refractive index between deuterated and normal solvents have been determined using a conventional gel permeation chromatograph equipped with a differential refractometer detector. Three molecular systems have been studied, namely, water–deuterium oxide, acetone–acetone (d6), and tetrahydrofuran–tetrahydrofuran (d8). A plot of the signal intensity versus the concentration of the deuterated solvent in the normal solvent gave a linear relationship for all three systems studied. The effect of deuteration is found to result in a lowering of the refractive index which is at least three orders of magnitude larger than the sensitivity of the differential refractometer. Consequently, for permeation chromatographic studies, the addition of 1% or less of the deuterated solvent in the injection sample would be more than sufficient to produce a peak characteristic of the mobile phase.