Immobilization of microbial cells on cellulose–polymer surfaces by radiation polymerization
Article first published online: 9 MAR 2003
Copyright © 1983 John Wiley & Sons, Inc.
Journal of Applied Polymer Science
Volume 28, Issue 12, pages 3759–3765, December 1983
How to Cite
Kumakura, M. and Kaetsu, I. (1983), Immobilization of microbial cells on cellulose–polymer surfaces by radiation polymerization. J. Appl. Polym. Sci., 28: 3759–3765. doi: 10.1002/app.1983.070281216
- Issue published online: 9 MAR 2003
- Article first published online: 9 MAR 2003
- Manuscript Accepted: 19 JUL 1983
- Manuscript Received: 12 MAY 1983
Streptomyces phaeochromogenes cells were immobilized on cellulose–polymer surfaces by radiation polymerization using hydrophilic monomers and paper. The enzyme activity of immobilized cell sheets was higher than that of immobilized cell composites obtained by the usual radiation polymerization technique. The enzyme activity of the sheets was affected by monomer concentration, the thickness of paper, and the degree of polymerization of paper. The copolymerization of hydroxyethyl methacrylate and methoxytetraethyleneglycol methacrylate in the sheets led to a further increase of the enzyme activity due to the increase of the hydrophilicity of the polymer matrix. The Michaelis constant of the sheets from low monomer concentration was close to that of intact cells.