Effective immobilization of protein linked with polyethylene glycol on silica via hydrogels using silica sol

Authors

  • Kohji Yoshinaga,

    Corresponding author
    1. Department of Chemistry, Kyushu Institute of Technology, Sensui, Tobata-ku, Kitakyushu 804, Japan
    • Department of Chemistry, Kyushu Institute of Technology, Sensui, Tobata-ku, Kitakyushu 804, Japan
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  • Taketoshi Kito,

    1. Department of Chemistry, Kyushu Institute of Technology, Sensui, Tobata-ku, Kitakyushu 804, Japan
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  • Makoto Yamaye

    1. Faculty of Engineering, Kyushu Kyoritsu University, Orio, Yahatanishi-ku, Kitakyushu 807, Japan
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Abstract

An effective attachment of activated polyethylene glycol (PEG), which has dichloro-s-triazinyl functional groups, to silica and protein immobilization with the resulting PEG/SiO2 composite were studied. The reaction of methanol—silica sol with the PEG—silyl reagent resulted in an efficient PEG attachment to SiO2 as compared with the usual two-step PEG modification of silica gel and glass surface. The activated PEG/SiO2 was able to immobilize over 600 mg of bovine serum albumin per unit gram of the PEG composite through hydrogel formation. The application of the present binding method of PEG-linked protein on SiO2 was also discussed.

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