Mansoor M. Amiji is now at Northeastern University, Department of Pharmaceutical Sciences, Boston, MA 02115.
Analysis on the surface adsorption of PEO/PPO/PEO triblock copolymers by radiolabelling and fluorescence techniques
Article first published online: 10 MAR 2003
Copyright © 1994 John Wiley & Sons, Inc.
Journal of Applied Polymer Science
Volume 52, Issue 4, pages 539–544, 25 April 1994
How to Cite
Amiji, M. M. and Park, K. (1994), Analysis on the surface adsorption of PEO/PPO/PEO triblock copolymers by radiolabelling and fluorescence techniques. J. Appl. Polym. Sci., 52: 539–544. doi: 10.1002/app.1994.070520409
- Issue published online: 10 MAR 2003
- Article first published online: 10 MAR 2003
- Manuscript Accepted: 14 OCT 1993
- Manuscript Received: 17 JUN 1993
We have examined the adsorption of poly(ethylene oxide)/ poly(propylene oxide)/poly(ethylene oxide) (PEO/PPO/PEO) triblock copolymers (Pluronics™) on dimethyldichlorosilane-treated glass (DDS-glass). The surface concentration of 125I-labeled Pluronic F-68(76/30/76) reached a maximum of 0.3 μg/cm2 when the bulk concentration in the adsorption solution was 3.0 mg/mL. Above 5.0 mg/ml, the surface Pluronic F-68 concentration started to decrease and reached 0.17 μg/cm2 when the bulk concentration for adsorption was 10 mg/mL. The surface concentration of Pluronic F-108 (129/56/129), on the other hand, increased to 4.0 μg/cm2 at the same bulk concentration. Fluroscence spectroscopic studies using pyrene suggested that the Pluronic F-68 molecules self-associated at the bulk concentration of 5.0 mg/mL and above. Because the aggregates are expected to expose the hydrophilic PEO segments to water, they may have lower affinity to DDS-glass. Aggregation of Pluronic F-68 also decreases the number of individual Pluronic molecules for adsorption. Pyrene fluorescence in Pluronic F-108 solution, however, suggests that Pluronic F-108 molecules do not form aggregates. It appears that the high surface concentrations of Pluronic F-108 may result from the preferential adsorption of individual molecules in multilayers. This explains the high effectiveness of Pluronic F-108 in preventing protein adsorption and platelet adhesion when adsorbed on to be hydrophobic surface. © 1994 John Wiley & Sons, Inc.