Development of an amperometric biosensor based on a redox-mediator-doped polypyrrole film

Authors

  • Rajesh,

    Corresponding author
    1. Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-Ku, Kitakyushu 808-0196, Japan
    • Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-Ku, Kitakyushu 808-0196, Japan
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  • Shyam S. Pandey,

    1. Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-Ku, Kitakyushu 808-0196, Japan
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  • W. Takashima,

    1. Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-Ku, Kitakyushu 808-0196, Japan
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  • K. Kaneto

    1. Graduate School of Life Science and System Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-Ku, Kitakyushu 808-0196, Japan
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Abstract

An amperometric biosensor was developed for the quantitative estimation of phenolic compounds in aqueous media. The enzyme tyrosinase [poly(phenol oxidase) (PPO)] was adsorbed onto a hexacyanoferrate(II)-ion-doped conducting polypyrrole (PPY) film deposited on an indium tin oxide (ITO) coated glass-plate support. The PPO activity in the PPO/Fe2+-PPY/ITO film was assayed as a function of the concentration of phenolic compounds. Cyclic voltammetric studies were carried out on this enzyme electrode, and the surface morphology of the enzyme-immobilized polymer film was studied with scanning electron microscopy. The results of the amperometric response of the PPO/Fe2+-PPY/ITO film showed sensitivities of 0.14, 0.21, and 0.36 A M−1cm−2 and linear response ranges of 9.9–84.7, 6.7–72.6, and 3.9–48.8 μM for phenol, catechol, and p-chlorophenol, respectively. The PPO/Fe2+-PPY/ITO electrode exhibited a response time of about 50 s and was stable for about 12 weeks at 4°C. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 927–933, 2004

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