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Gel permeation chromatography calibration based on fractal geometry

Authors

  • A. G. Boborodea,

    Corresponding author
    1. Unité de Physique et de Chimie des Hauts Polymères, Université catholique de Louvain, Bâtiment Boltzmann, Place Croix du Sud, 1 B-1348 Louvain-la-Neuve, Belgium
    • Unité de Physique et de Chimie des Hauts Polymères, Université catholique de Louvain, Bâtiment Boltzmann, Place Croix du Sud, 1 B-1348 Louvain-la-Neuve, Belgium
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  • M. Clemens,

    1. Department of Chemistry and Materials, Manchester Metropolitan University, John Dalton Building, Chester Street, Manchester, M1 5GD, UK
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  • D. Daoust,

    1. Unité de Physique et de Chimie des Hauts Polymères, Université catholique de Louvain, Bâtiment Boltzmann, Place Croix du Sud, 1 B-1348 Louvain-la-Neuve, Belgium
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  • C. Bailly

    1. Unité de Physique et de Chimie des Hauts Polymères, Université catholique de Louvain, Bâtiment Boltzmann, Place Croix du Sud, 1 B-1348 Louvain-la-Neuve, Belgium
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Abstract

The previously developed model [Polym Bull 2000, 44, 525] used to characterize the porous gel inside a gel permeation chromatography (GPC) column, has been extended to also include the interstitial space between the macroscopic gel particles. The hydrodynamic dimensions for 12 polystyrene (PS) standards, measured by GPC with differential refractive index (DRI), differential viscometry (VISCO), and multiangle laser light scattering (MALLS) detectors, have been used to determine the fractal parameters of the polystyrene–divinylbenzene gel corresponding to four commercial columns. The new developed model enables to predict the calibration curve for the sets of coupled columns based on the parameters of each column. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 771–777, 2004

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