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DNA-loaded porous polyethersulfone particles for environmental applications I. preparation

Authors

  • Changsheng Zhao,

    Corresponding author
    1. Department of Biopolymer Materials, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, People's Republic of China
    • Department of Biopolymer Materials, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, People's Republic of China
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  • Shudong Sun,

    1. Department of Biopolymer Materials, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, People's Republic of China
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  • Kaiguang Yang,

    1. Department of Biopolymer Materials, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, People's Republic of China
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  • Motoyoshi Nomizu,

    1. Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan
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  • Norio Nishi

    1. Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan
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Abstract

DNA-loaded porous poly(ether sulfone) (PES) particles are prepared for environmental applications by means of a liquid–liquid phase separation technique. The particles prepared by this method have very large porosity and specific surface area, which are useful as absorbents. By manipulating the fabrication conditions, we could control the microsphere structure, including the diameter and porosity of the particles, the pore size, and the specific surface area. Increasing the polymer concentration, which causes lower porosity and smaller pores on the outer surface of the microspheres, led to increased stability of the DNA-loaded particles and high DNA incorporation efficiency. The DNA-loaded porous PES particles could accumulate and remove a DNA intercalating pollutant, ethidium bromide (EB). Both the DNA amount incorporated in the particles and the microsphere structure have great effect on the EB removal ratio. These results indicate that the DNA-loaded PES particles have the potential to be used in environmental applications, which will be further discussed in the following paper. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 1668–1673, 2005

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