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Cytotoxicity and hemocompatibility of a family of novel MeO-PEG-poly (D,L-lactic-co-glycolic acid)-PEG-OMe triblock copolymer nanoparticles

Authors

  • Lili He,

    1. Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Sichuan Chengdu 610041, China
    2. College of Chemistry and Environment Protection Engineering, Southwest University for Nationalities, Sichuan, Chengdu 610041, China
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  • Likai Yang,

    1. Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Sichuan Chengdu 610041, China
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  • Yourong Duan,

    1. Shanghai Cancer Institute, Cancer Institute of Shanghai JiaoTong University, Shanghai 200032, China
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  • Li Deng,

    1. Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Sichuan Chengdu 610041, China
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  • Xun Sun,

    1. Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Sichuan Chengdu 610041, China
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  • Zhongwei Gu,

    1. National Engineering Research Center for Biomaterials, Sichuan University, Sichuan Chengdu 610041, China
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  • Zhi-Rong Zhang

    Corresponding author
    1. Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Sichuan Chengdu 610041, China
    • Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Sichuan Chengdu 610041, China
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Abstract

A family of newly synthesized monomethoxy (polyethylene glycol)-poly (D,L-lactic glycolic acid)- monomethoxy(polyethylene glycol) (MeO-PEG-poly (D,L-lactic-co-glycolic acid)-PEG-OMe, PELGE) biodegradable polymers are candidates for intravenous nanoparticle drug, because of their merits of biocompatibility and blood compatibility, and their capability of escaping from the endothelium system (RES) and adsorbing proteins. In the current research, relationships between composition, cytotoxicity, and hemocompatibility of a series of blank PELGE nanoparticles were investigated. Cytotoxicity on Chang cell lines was investigated using the methyl thiazolyl tetrazolium (MTT) assay. Human and rabbit blood were used in studies of red blood cell hemolysis, whole blood clotting time, plasma recalcification profiles, and red blood cell form and appearance in whole blood. The results suggested that the molecular weight of PEG used in the synthesis of polymers influenced their characteristics. Generally, as the molecular weight of PEG increased, increased cytotoxicity and hemocompatibility were observed. The RGR (relative growth rate) of PELGE nanoparticles synthesized with PEG 550 was above 70%, while that of PELGE nanoparticles synthesized with PEG 750 and PEG 2000 was in the range of 55–105% and 36–87% respectively. For PELGE nanoparticles synthesized with PEG 550, most hemolysis values were in the range of 1–3%, while for PELGE nanoparticles synthesized with PEG 750 and PEG 2000 hemolysis values were 1–2% and below 2%, respectively. None of the nanoparticles caused changes in red blood cell form or appearance. Based on the results, 12 kinds of PELGE were chosen for further studies. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci 113: 2933–2944, 2009

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