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Keywords:

  • Zr4+;
  • affinity column;
  • atomic transfer radical polymerization;
  • Wang resin;
  • phosphonated casein;
  • resolution time

Abstract

This paper describes the preparation of the metallic affinity column by atom transfer radical polymerization of glycidyl methacrylate (GMA) on Wang resin surface. The metallic ions (Zr4+, Fe3+, and Pd2+) were introduced after phosphonation of the epoxy group of the grafted poly(GMA) on Wang resin surface. The successful results on metallic ion-immobilized polymeric microsphere stationary phase were confirmed via Fourier transform infrared, scanning electron microscopy, X-ray photoelectron spectra (XPS), and inductively coupled plasma-atomic emission spectrometer. The resolution degree of the phosphonated casein and dephosphonated casein for Zr4+, Fe3+, and Pd2+ affinity column was examined. The result shows that the resolution time (min) of the phosphonated casein was higher than that of the dephosphated casein on the Zr4+ affinity column by liquid chromatography. However, the phosphonated casein and dephosphonated casein were not separated on the Fe3+ affinity column and Pd2+ affinity column by liquid chromatography. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009