• biomaterials;
  • bioengineering;
  • polyurethanes;
  • separation techniques;
  • hematopoietic stem cells


The separation of hematopoietic stem and progenitor cells (CD34+ cells) from peripheral blood was investigated using foamed polyurethane (PU) membranes modified with several amino acids. CD34+ cells were collected by first allowing the blood to permeate through the membranes, and then passing the recovered solution through the membranes. Optimal conditions for the separation of CD34+ cells were investigated. The highest recovery ratio of CD34+ cells was obtained using three sheets of PU membranes having carboxylic acid groups (PU[BOND]COOH) modified with glycine, the membranes having been pretreated by immersion in phosphate buffer solution prior to permeation of blood. A high recovery ratio of CD34+ cells was achieved in a recovery process using 0.5 wt % human serum albumin (HSA) or 20% dextran solution passed through PU[BOND]COOH membranes. The recovery ratios of CD34+ cells using platelet-poor plasma and platelet-rich plasma were approximately 20% and 30%, respectively, significantly less than the ratio found using 0.5 wt % HSA solution. Surface-modified membranes having carboxylic acid groups showed a higher recovery ratio of CD34+ cells than membranes having zwitterionic groups. The effect of carboxylic acid groups on the surface-modified PU membranes was to generate weak interactions by electrostatic repulsion between CD34+ cells and the membranes because of the negatively charged surfaces of the cells, allowing them to be detached from the membranes and collected in the recovered solution. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009