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Suppression of breast cancer cells in vitro by polyamidoamine-dendrimer-mediated 5-fluorouracil chemotherapy combined with antisense micro-RNA 21 gene therapy

Authors

  • Mei Mei,

    1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
    2. Tianjin Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China
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  • Yu Ren,

    1. Tianjin Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China
    2. School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China
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  • Xuan Zhou,

    1. First Department of Head and Neck Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
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  • Xu-Bo Yuan,

    1. School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China
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  • Fei Li,

    1. School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China
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  • Ling-Huo Jiang,

    1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
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  • Chun-Sheng Kang,

    Corresponding author
    1. Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, China
    • Department of Neurosurgery, Tianjin Medical University General Hospital, Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052, China
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  • Zhi Yao

    Corresponding author
    1. Department of Immunology, Tianjin Medical University, Tianjin 300070, China
    • Department of Immunology, Tianjin Medical University, Tianjin 300070, China
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Abstract

A specific micro-RNA (miRNA), micro-RNA 21 (miR-21), is strongly overexpressed in breast cancer cells. Antisense inhibition of miRNA function, an important tool for uncovering miRNA biology, which is often used to knockdown miRNA, can cause a notable inhibition of cell growth. In this study, 5-fluorouracil (5-FU) was conjugated to polyamidoamine dendrimers via direct encapsulation; this method was then combined with antisense micro-RNA 21 (as-miR-21) strategies to evaluate the effects of the growth suppression of breast cancer cells. Our results show that as-miR-21 strategies significantly improved the chemosensitivity of free 5-FU on breast cancer cells (MCF-7). In addition, not only could as-miR-21 effectively increase the apoptotic cell numbers but it could also bring down the migration ability of MCF-7 cells. Our results provide invaluable information for the future design of drug–polymer complexes for multimodal cancer treatments. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009

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