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Immobilization of alcohol dehydrogenase on films prepared by the electrochemical copolymerization of pyrrole and 1-(2-carboxyethyl)pyrrole for ethanol sensing

Authors

  • Masato Shimomura,

    Corresponding author
    1. Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology, 1603-1, Kamitomioka-Machi, Nagaoka 940-2188, Japan
    • Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology, 1603-1, Kamitomioka-Machi, Nagaoka 940-2188, Japan
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  • Takashi Kuwahara,

    1. Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology, 1603-1, Kamitomioka-Machi, Nagaoka 940-2188, Japan
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  • Kaede Iizuka,

    1. Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology, 1603-1, Kamitomioka-Machi, Nagaoka 940-2188, Japan
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  • Toshiki Kinoshita

    1. Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology, 1603-1, Kamitomioka-Machi, Nagaoka 940-2188, Japan
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Abstract

The electrochemical copolymerization of pyrrole and 1-(2-carboxyethyl)pyrrole (Py-COOH) was carried out, and a conducting polymer film with a Py-COOH content of 5% and a conductivity of 6.6 × 10−4 S/cm was obtained. Alcohol dehydrogenase (ADH) was attached through amide linkage onto the surface of the conducting polymer film for the purpose of fabricating an ADH-immobilized electrode applicable to the amperometric sensing of ethanol. The quantity and activity of the immobilized ADH were determined to be 400 μg/cm2 and 0.6 U/mg, respectively. With the ADH-immobilized electrode, amperometric ethanol sensing was attempted in the presence of nicotinamide adenine dinucleotide (a cofactor of ADH) and Meldola's blue (an electron-transferring mediator). The ADH-immobilized electrode made the current response correspond to ethanol concentration, which reached 10 μA/cm2 at a concentration of 14 mM. The selectivity of the sensing was examined with alcohols other than ethanol. It was found that the ADH/copolymer electrode had a considerably larger current response to allyl alcohol. The selectivity was attributed to the specificity of native ADH with respect to the oxidation of alcohols. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010

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