Malic enzyme (EC 126.96.36.199) and alanine dehydrogenase (EC 188.8.131.52) were entrap-immobilized on hybrid gel fibers of cellulose acetate (CA) and zirconium (Zr) alkoxide by air-gap wet spinning. The production of L-alanine from malic acid with coenzyme regeneration was examined with the enzymes immobilized on the fibers. The productivity of L-alanine of the immobilized enzymes decreased to approximately one-fifth of that of free enzymes, but the CA–Zr-fiber-immobilized enzymes retained a high level of productivity after repeated use. Reduced form of nicotinamide adenine dinucleotide (NADH) recycling also occurred effectively for the enzymes immobilized on the fiber. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
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