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Coimmobilization of malic enzyme and alanine dehydrogenase on organic–inorganic hybrid gel fibers and the production of L-alanine from malic acid using the fibers with coenzyme regeneration

Authors

  • Koji Nakane,

    Corresponding author
    1. Materials Science and Engineering Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
    • Materials Science and Engineering Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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  • Shin-Ichiro Suye,

    1. Applied Chemistry and Biotechnology Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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  • Tomoya Ueno,

    1. Materials Science and Engineering Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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  • Yoko Ohno,

    1. Applied Chemistry and Biotechnology Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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  • Tomoko Ishikawa,

    1. Applied Chemistry and Biotechnology Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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  • Takashi Ogihara,

    1. Materials Science and Engineering Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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  • Nobuo Ogata

    1. Materials Science and Engineering Course, Graduate School of Engineering, University of Fukui, Bunkyo 3-9-1, Fukui 910-8507, Japan
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Abstract

Malic enzyme (EC 1.1.1.39) and alanine dehydrogenase (EC 1.4.1.1) were entrap-immobilized on hybrid gel fibers of cellulose acetate (CA) and zirconium (Zr) alkoxide by air-gap wet spinning. The production of L-alanine from malic acid with coenzyme regeneration was examined with the enzymes immobilized on the fibers. The productivity of L-alanine of the immobilized enzymes decreased to approximately one-fifth of that of free enzymes, but the CA–Zr-fiber-immobilized enzymes retained a high level of productivity after repeated use. Reduced form of nicotinamide adenine dinucleotide (NADH) recycling also occurred effectively for the enzymes immobilized on the fiber. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010

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