Synthesis of hydroxyethylcellulose-graft-poly(N, N-dimethylacrylamide) copolymer by ATRP and as dynamic coating in capillary electrophoresis

Authors

  • Runmiao Yang,

    Corresponding author
    1. Department of Material Engineering, Jiangsu Teachers University of Technology, Changzhou 213001, People's Republic of China
    • Department of Material Engineering, Jiangsu Teachers University of Technology, Changzhou 213001, People's Republic of China
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  • Yuhai Liu,

    1. Department of Material Engineering, Jiangsu Teachers University of Technology, Changzhou 213001, People's Republic of China
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  • Chunzhi Zheng

    1. Department of Material Engineering, Jiangsu Teachers University of Technology, Changzhou 213001, People's Republic of China
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Abstract

Hydroxyethylcellulose-graft-poly (N, N-dimethylacrylamide) was synthesized by successive atom transfer radical polymerization (ATRP) of N,N-dimethylacrylamide (DMA) monomer using HEC-Br as initiator, CuBr and 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazamacrocyclotetradecane (Me6[14]aneN4) as catalyst and ligand, with molar ratio DMA: HEC-Br (C[BOND]Br): CuBr: Me6[14]aneN4 = 100 : 1 : 1 : 3. HEC–Br macroinitiator was synthesized by esterification of HEC with 2-bromoisobutyryl bromide. GPC and 1H NMR studies show that the molecular weight of the resulting PDMA increased linearly with the conversion. Within 6 h, the polymerization can reach almost 60% of conversion. The copolymer is applied for the separation of basic proteins in capillary electrophoresis. The results show that this medium has a powerful capability in resisting basic proteins adsorption because the polymer forms noncovalent coating in silica capillaries. With a broad range of pH 2–7, proteins were separated with sufficient efficiencies above 200,000 plates/m. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010

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