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Acetylation of dendrimer-entrapped gold nanoparticles: Synthesis, stability, and X-ray attenuation properties

Authors

  • Chen Peng,

    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People's Republic of China
    2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China
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  • Han Wang,

    1. Department of Radiology, Shanghai First People's Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200080, People's Republic of China
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  • Rui Guo,

    1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China
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  • Mingwu Shen,

    1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China
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  • Xueyan Cao,

    1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China
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  • Meifang Zhu,

    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People's Republic of China
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  • Guixiang Zhang,

    Corresponding author
    1. Department of Radiology, Shanghai First People's Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200080, People's Republic of China
    • Department of Radiology, Shanghai First People's Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200080, People's Republic of China
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  • Xiangyang Shi

    Corresponding author
    1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People's Republic of China
    2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China
    • State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, People's Republic of China
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Abstract

Functionalized dendrimer-entrapped gold nanoparticles (Au DENPs) are of scientific and technological interest in biomedical applications. In this study, Au DENPs prepared with amine-terminated generation 5 (G5) poly(amido amine) dendrimers as templates were subjected to acetylation to neutralize the positive surface charge of the particles. By varying the molar ratio of Au salt to G5 dendrimer, we prepared acetylated Au DENPs with a size range of 2–4 nm. Meanwhile, we attempted to add glucose to the dialysis liquid of the acetylated Au DENPs to prevent possible particle aggregation after lyophilization. The acetylated Au DENPs with different compositions (Au salt/dendrimer molar ratios) were characterized with 1H-NMR, transmission electron microscopy, ultraviolet–visible (UV–vis) spectrometry, and ζ-potential measurements. We show that when the molar ratio of Au salt to dendrimer was equal to or larger than 75:1, the acetylated Au DENPs showed a significant aggregation after lyophilization, and the addition of glucose was able to preserve the colloidal stability of the particles. X-ray absorption measurements showed that the attenuation of the acetylated Au DENPs was much higher than that of the iodine-based contrast agent at the same molar concentration of the active element (Au vs iodine). In addition, the acetylated Au DENPs enabled X-ray computed tomography (CT) imaging of mice after intravenous injection of the particles. These findings suggest a great potential for acetylated Au DENPs as a promising contrast agent for CT imaging applications. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

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