Synthesis, characterization, and drug-release behavior of novel PEGylated bovine serum albumin as a carrier for anticancer agents

Authors

  • Soodabeh Davaran,

    1. Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
    2. School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
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  • Davoud Asgari,

    1. School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
    2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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  • Mohammad Reza Rashidi,

    1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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  • Roya Salehi,

    1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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  • Yadolah Omidi

    Corresponding author
    1. Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
    2. School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
    • Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
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Abstract

To develop a novel pH-sensitive PEGylated carrier for protein-based anticancer agents, we modified bovine serum albumin (BSA) with poly(ethylene glycol) citrate ester (PEG–CA) through amidation with its amino groups. Increasing the mixing ratio of albumin from 3 to 6 with respect to PEG–CA resulted in a 2-fold increase in the degree of albumin modification. Adriamycin (ADR)-loaded PEG–CA–BSA hydrogels and microparticles were prepared, and the cumulative amounts of ADR released from the PEG–CA–BSA hydrogels (phosphate-buffered saline, pH 7.4) showed that all the PEG-CA-BSA(x) (x represents degree of substitution of PEG to amino group of albumin, i.e. 26%, 28%, 31% and 49%) hydrogels had lower ADR release rates with a slight initial burst release. During the first 24 h, the cumulative releases were 15.5% for PEG–CA–BSA(49), 24% for PEG–CA–BSA(31), 31% for PEG–CA–BSA(28), and 38% for PEG–CA–BSA(26). Afterward, all the release rates slowed, and they were almost in the following order: PEG–CA–BSA(26) > PEG–CA–BSA(28) > PEG–CA–BSA(31) > PEG–CA–BSA(49). The release rates of ADR from the microparticles were dependent on the amount of glutaraldehyde. According to our findings, a higher PEG–CA/BSA molar ratio led to a reduced cumulative amount of ADR released from the hydrogels, whereas higher release rates were observed for microparticles with a lower amount of BSA in the conjugates in a pH-dependent manner. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

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