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Keywords:

  • urease;
  • immobilization;
  • proton conductivity;
  • polymernNetwork

Abstract

In this article, urease was immobilized in a conducting network via complexation of poly(1-vinyl imidazole) (PVI) with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS). The preparation method for the polymer network was adjusted by using Fourier transform infrared (FTIR) spectroscopy. A scanning electron microscope (SEM) study revealed that enzyme immobilization had a strong effect on film morphology. The proton conductivity of the PVI/PAMPS network was measured via impedance spectroscopy, under humidified conditions. The basic characteristics (Michealis-Menten constants, pHopt, pHstability, Topt, Tstability, reusability, and storage stability) of the immobilized urease were determined. The obtained results showed that the PAA/PVI polymer network was suitable for enzyme immobilization. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011