Shanqin Xu and Xiaoli Yan contributed equally to this work.
1097-4628/asset/olbannerleft.gif?v=1&s=cee22a07a3decc25a0270f06ff729dd397546eea)
In vitro biocompatibility of electrospun silk fibroin mats with Schwann cells
Article first published online: 29 SEP 2010
DOI: 10.1002/app.32996
Copyright © 2010 Wiley Periodicals, Inc.
Additional Information
How to Cite
Xu, S., Yan, X., Zhao, Y., Wang, W. and Yang, Y. (2011), In vitro biocompatibility of electrospun silk fibroin mats with Schwann cells. J. Appl. Polym. Sci., 119: 3490–3494. doi: 10.1002/app.32996
- †
Shanqin Xu and Xiaoli Yan contributed equally to this work.
Publication History
- Issue published online: 3 DEC 2010
- Article first published online: 29 SEP 2010
- Manuscript Accepted: 24 JUN 2010
- Manuscript Received: 28 DEC 2009
Funded by
- Hi-Tech Research and Development Program of China (863 Program). Grant Number: 2006AA02A128
- Nature Science Foundation of China. Grant Numbers: 30770585, 30970713
- Nature Science Foundation of Jiangsu Province. Grant Number: BK2009518
- Program for New Century Excellent Talents in University
- Abstract
- Article
- References
- Cited By
Keywords:
- biocompatibility biomaterials;
- nanofiber
Abstract
In this study, electrospinning was used to fabricate silk-fibroin (SF)-based mats, which served as substrates for the culturing of rat Schwann cells. Microscopic observation and physical parameter measurements revealed that the electrospun SF mats had a nanofibrous structure with favorable physical properties. Fourier transform infrared analysis provided chemical characterization of the molecular confirmation of the SF proteins in the mats. The morphology and immunocytochemistry showed that the mats supported the survival and growth of the cultured Schwann cells, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis indicated that the electrospun SF mat extract had no cytotoxic effects on Schwann cell proliferation. Collectively, all of the results suggest that the electrospun SF mats might become a candidate scaffold for tissue-engineered nerve grafts to promote peripheral nerve regeneration. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011