Advertisement

In vitro biocompatibility of electrospun silk fibroin mats with Schwann cells

Authors

  • Shanqin Xu,

    1. School of Textile and Clothing, Nantong University, 19 Qixiu Road, Nantong, JS 226001, China
    Search for more papers by this author
    • Shanqin Xu and Xiaoli Yan contributed equally to this work.

  • Xiaoli Yan,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, China
    Search for more papers by this author
    • Shanqin Xu and Xiaoli Yan contributed equally to this work.

  • Yahong Zhao,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, China
    Search for more papers by this author
  • Wei Wang,

    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, China
    Search for more papers by this author
  • Yumin Yang

    Corresponding author
    1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, China
    • Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS 226001, China
    Search for more papers by this author

Abstract

In this study, electrospinning was used to fabricate silk-fibroin (SF)-based mats, which served as substrates for the culturing of rat Schwann cells. Microscopic observation and physical parameter measurements revealed that the electrospun SF mats had a nanofibrous structure with favorable physical properties. Fourier transform infrared analysis provided chemical characterization of the molecular confirmation of the SF proteins in the mats. The morphology and immunocytochemistry showed that the mats supported the survival and growth of the cultured Schwann cells, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis indicated that the electrospun SF mat extract had no cytotoxic effects on Schwann cell proliferation. Collectively, all of the results suggest that the electrospun SF mats might become a candidate scaffold for tissue-engineered nerve grafts to promote peripheral nerve regeneration. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Ancillary