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Multifunctional gentamicin supplementation of poly(γ-glutamic acid)-based hydrogels for wound dressing application

Authors

  • Yu-Hsin Lin,

    Corresponding author
    1. Department of Biological Science and Technology, Center for Inflammation Research, China Medical University, Taichung, Taiwan, Republic of China
    • Department of Biological Science and Technology, Center for Inflammation Research, China Medical University, Taichung, Taiwan, Republic of China
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  • Jui-Hsiang Lin,

    1. Department of Materials Science and Engineering, Feng Chia University, Taichung, Taiwan, Republic of China
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  • Shu-Fen Peng,

    1. Department of Biological Science and Technology, Center for Inflammation Research, China Medical University, Taichung, Taiwan, Republic of China
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  • Chia-Lin Yeh,

    1. Department of Biological Science and Technology, Center for Inflammation Research, China Medical University, Taichung, Taiwan, Republic of China
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  • Wen-Chen Chen,

    1. Department of Biological Science and Technology, Center for Inflammation Research, China Medical University, Taichung, Taiwan, Republic of China
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  • Tsai-Luan Chang,

    1. Department of Applied Cosmetology and Graduate Institute of Cosmetic Science, Hungkuang University, Taichung, Taiwan, Republic of China
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  • Ming-Ju Liu,

    1. Department of Applied Cosmetology and Graduate Institute of Cosmetic Science, Hungkuang University, Taichung, Taiwan, Republic of China
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  • Chih-Ho Lai

    1. Department of Microbiology, School of Medicine, China Medical University, Taichung, Taiwan, Republic of China
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Abstract

The process of wound healing is composed of coagulation, inflammation, fibroplasia, collagenation, epithelization, and wound contraction. The wound dressing should protect the wound from bacterial infection, maintain a moist healing environment, and promote cell migration to reconstruct damaged tissue, and be easy to apply and remove to improve patient comfort. The purpose of our study was to develop multifunctional hydrogels composed of genipin-crosslinked biodegradable biomaterials of poly(γ-glutamic acid) and gelatin, encapsulating gentamicin to accelerate wound healing. The results of swelling ratio measurements clearly indicate that hydrogel composition of poly(γ-glutamic acid)–gelatin had a higher swelling ratio and lower peel adhesion properties than gelatin hydrogel alone. In an in vitro study, the gentamicin incorporated in prepared hydrogels effectively inhibited target microorganisms and produced a higher expression of Type I collagen in fibroblast cells. Confocal laser scanning microscopy revealed that the fibroblast cells cultured in the hydrogel membranes produced fibroblast cell migration and showed a continuous lined cytoskeletal distributing status. In the in vivo study, it was found that the gentamicin incorporated in genipin-crosslinked γ-PGA–gelatin wound dressing demonstrates the potential of such biologically functionalized dressing to accelerate wound closure and, hence, its potential clinical usefulness. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

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