Electrochemical immobilization of ascorbate oxidase in poly(3,4-ethylenedioxythiophene)/multiwalled carbon nanotubes composite films

Authors

  • Ming Liu,

    1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education and Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China
    2. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Yangping Wen,

    1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education and Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China
    2. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Dong Li,

    1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education and Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China
    2. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Haohua He,

    Corresponding author
    1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education and Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China
    • Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education and Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China
    Search for more papers by this author
  • Jingkun Xu,

    Corresponding author
    1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    • Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Congcong Liu,

    1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Ruirui Yue,

    1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Baoyang Lu,

    1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author
  • Guodong Liu

    1. School of Communication and Electronics, Jiangxi Science and Technology Normal University, Nanchang 330013, China
    Search for more papers by this author

Abstract

Immobilization of ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT)/multiwalled carbon nanotubes (MWCNTs) composite films was achieved by one-step electrochemical polymerization. The PEDOT/MWCNTs/AO modified electrode was fabricated by the entrapment of enzyme in conducting matrices during electrochemical polymerization. The PEDOT/MWCNTs modified electrodes were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The experimental results showed that the composite films exhibited better mechanical integrity, electrochemical activity, higher electronic and ionic conductivity, and larger redox capacitance compared with pure PEDOT films, which would be beneficial to the fabrication of PEDOT/MWCNTs/AO electrochemical biosensors. The scanning electron microscopy studies revealed that MWCNTs served as backbone for 3,4-ethylenedioxythiophene (EDOT) electropolymerization. Furthermore, the resulting enzyme electrode could be used to determine L-ascorbic acid successfully, which demonstrated the good bioelectrochemical catalytic activity of the immobilized AO. The results indicated that the PEDOT/MWCNTs composite are a good candidate material for the immobilization of AO in the fabrication of enzyme-based biosensor. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Ancillary