These authors contributed equally to this work.
Synergistic inhibition of human glioma cell line by temozolomide and PAMAM-mediated miR-21i
Article first published online: 27 APR 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Applied Polymer Science
Volume 127, Issue 1, pages 570–576, 5 January 2013
How to Cite
Qian, X.-M., Shi, Z.-D., Ren, Y., Liu, C.-Y., Ji, Y.-R., Long, L.-X., Pu, P., Sheng, J., Yuan, X.-B. and Kang, C.-S. (2013), Synergistic inhibition of human glioma cell line by temozolomide and PAMAM-mediated miR-21i. J. Appl. Polym. Sci., 127: 570–576. doi: 10.1002/app.37823
- Issue published online: 8 OCT 2012
- Article first published online: 27 APR 2012
- Manuscript Accepted: 30 MAR 2012
- Manuscript Received: 16 SEP 2011
- National Nature Science Foundation of China. Grant Numbers: 51073118, 51103107, 81172406
- Program for New Century Excellent Talents in University. Grant Number: NCET-08-0393)
- MiR-21 inhibitor;
Temozolomide (TMZ) is a promising chemotherapeutic agent for treating glioblastomas. However, resistance develops quickly and with a high frequency. Efforts to overcome chemoresistance are, therefore, critically needed. In present study, a poly(amidoamine; PAMAM) dendrimer was used as a vector to deliver microRNA-21 inhibitor (miR-21i) into U87 cells and the chemosensitivity of the combination effect of miR-21i and TMZ for glioma therapy was investigated. Flow cytometry analysis showed the uptake efficiency of microRNA-21 inhibitor after complexation with PAMAM. Real-time PCR and in situ hybridization indicated that, compared with TMZ or miR-21i treated cells, cells simultaneously treated with miR-21i and TMZ showed a remarkable decrease in the microRNA-21 (miR-21) level. The transfection of miR-21i enhanced the chemosensitivity by significantly decreasing the IC50 value of TMZ to glioma cells. Knockdown of miR-21 promoted the cells' apoptosis, and at the same time, inhibited cell invasion. In conclusion, the combination treatment of glioma cells with TMZ and miR-21i could yield a synergistic effect in inhibition of human glioma cell line. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013