Structural, mechanical, and biocompatibility analyses of a novel dental restorative nanocomposite

Authors

  • A. S. Khan,

    1. Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology, Lahore, Pakistan
    2. School of Engineering and Materials Science, Queen Mary University of London, London, United Kingdom
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  • F. S. L. Wong,

    1. Paediatric Dentistry, Centre for Oral Growth and Development, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
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  • I. J. McKay,

    1. Clinical and Diagnostic Oral Sciences, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
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  • R. A. Whiley,

    1. Clinical and Diagnostic Oral Sciences, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
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  • I. U. Rehman

    Corresponding author
    1. Department of Material Science and Engineering, The Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield S3 7HQ, United Kingdom
    • Department of Material Science and Engineering, The Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield S3 7HQ, United Kingdom
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Abstract

Structure and biocompatibility are key parameters that determine the usefulness of dental materials for clinical use. Novel polyurethane (PU) nanocomposite material was prepared by chemically binding nanohydroxyapatite (nHA) to the diisocyanate component of the PU backbone by solvent-polymerization. nHA was incorporated into PU by the stepwise addition of monomeric units of the PU. The PU/nHA composite was analyzed by 13C Nuclear magnetic resonance (structural) and X-ray diffraction (phase analysis). The tensile strength and elastic modulus was evaluated for mechanical properties. These analyses revealed linkage between the hard- and soft-segments are urethane linkage and showed high mechanical properties with increase in content of nHA. To assess biocompatibility osteoblast cells were seeded on to the material and allowed to adhere and proliferate. Osteoblast-like cell growth and proliferation was assessed by MTS assay. It was found that cells adhered and proliferated on these novel substrates. To test bacterial adhesion discs of composite with and without nHA were incubated with standardized suspensions of oral bacterium Streptococcus sanguinis strain NCTC 7863. PU composites with nHA exhibited biocompatibility with respect to mammalian cell growth and showed significantly reduced bacterial adhesion as compared to PU alone. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

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