Advertisement

Synthesis of hydroxyethylcellulose-g-methoxypoly (ethylene glycol) copolymer and its application for protein separation in CE

Authors

  • Xiaohua Shi,

    1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    Search for more papers by this author
  • Lin Tan,

    1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    Search for more papers by this author
  • Jinxing Xing,

    1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    Search for more papers by this author
  • Fuhu Cao,

    1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    Search for more papers by this author
  • Lijuan Chen,

    1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    Search for more papers by this author
  • Zhaofeng Luo,

    1. Instrument Center of Life Science, University of Science and Technology of China, Hefei 230027, People's Republic of China
    Search for more papers by this author
  • Yanmei Wang

    Corresponding author
    1. CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    • CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, People's Republic of China
    Search for more papers by this author

Abstract

Hydroxyethylcellulose-g-methoxypoly (ethylene glycol) (HEC-g-PEG) graft copolymers were synthesized through the etherification reaction between the hydroxyl group of hydroxyethylcellulose (HEC) and iodinated methoxypoly (ethylene glycol) (PEG-I), which was prepared on the basis of two-step reaction. Fourier transforms infrared spectrum (FTIR), nuclear magnetic resonance (NMR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and iodide oxidation method were used to prove the success of synthesis of graft copolymer. Furthermore, the comparative studies of electro-osmotic flow (EOF) and protein separation in bare-fused silica, HEC and HEC-g-PEG-coated capillary were performed in capillary electrophoresis (CE). The results showed that HEC-g-PEG-coated capillary presented efficient EOF suppression ability and excellent resisting protein adsorption ability. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

Ancillary