Production of 3D scaffolds applied to tissue engineering using chitosan swelling as a porogenic agent

Authors

  • Roberta Helena Mendonça,

    1. PEMM/COPPE, Universidade Federal do Rio de Janeiro, Ilha do Fundão, PO Box 68505, 21941-972, Rio de Janeiro, RJ, Brazil
    2. DEQ/IT, Universidade Federal Rural do Rio de Janeiro, Rod BR 465, 23890-000, Seropédica, RJ, Brazil
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  • Taíla de Oliveira Meiga,

    1. PEMM/COPPE, Universidade Federal do Rio de Janeiro, Ilha do Fundão, PO Box 68505, 21941-972, Rio de Janeiro, RJ, Brazil
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  • Marysilvia Ferreira da Costa,

    Corresponding author
    1. PEMM/COPPE, Universidade Federal do Rio de Janeiro, Ilha do Fundão, PO Box 68505, 21941-972, Rio de Janeiro, RJ, Brazil
    • PEMM/COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil
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  • Rossana Mara da Silva Moreira Thiré

    1. PEMM/COPPE, Universidade Federal do Rio de Janeiro, Ilha do Fundão, PO Box 68505, 21941-972, Rio de Janeiro, RJ, Brazil
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Abstract

The preparation of 3D scaffolds using poly(3-hydroxybutyrate) (PHB) and chitosan (CTS) has been proposed because of the favorable interactions of these materials with mammalian cells in particular osteoblasts (HOB). In this work, a new method of producing porous 3D scaffolds based on the swelling of a dense matrix of PHB and CTS was developed. Scanning electron microscopy images showed the formation of an interconnected porous CTS structure inside a PHB structure. The pore formation was attributed to the swelling of CTS. The scaffolds presented 70% porosity, heterogeneous pore-size distribution, and a compressive modulus equal to (13.07 ± 0.014) MPa, which is close to that of cancellous bone. In vitro cytotoxicity assays indicate that the scaffold was not cytotoxic. Its morphology, chemical, and physical properties indicate that the scaffold produced by this new methodology has potential applications in bone-tissue engineering, with drug-delivery capabilities. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

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