Enhancing the stability of immobilized catalase on activated carbon with gelatin encapsulation

Authors

  • Yulin Wang,

    1. State Key Laboratory of Chemical Resource Engineering, The Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing, People's Republic of China
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    • The first two authors contributed equally to this work and should be considered co first authors.

  • Yuepeng Guan,

    1. Science College of Hunan Agricultural University, Changsha City, Hunan Province, People's Republic of China
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    • The first two authors contributed equally to this work and should be considered co first authors.

  • Yin Yang,

    1. State Key Laboratory of Chemical Resource Engineering, The Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing, People's Republic of China
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  • Peng Yu,

    Corresponding author
    • Science College of Hunan Agricultural University, Changsha City, Hunan Province, People's Republic of China
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  • Yaqin Huang

    Corresponding author
    • State Key Laboratory of Chemical Resource Engineering, The Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing, People's Republic of China
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Correspondence to: P. Yu (E-mail: yuzhiyipeng@163.com) or Y. Huang (E-mail: huangyaqin9@sina.com)

ABSTRACT

Gelatin (Gel) encapsulation onto activated carbon (AC) with catalase (CAT) was developed as an alternative method for CAT immobilization in this study. The immobilized CAT with AC encapsulated by Gel as the supporter accounted for 65.69% of the native enzyme activity. Furthermore, the properties of the immobilized CATs were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Among free CAT and the two immobilized CATs, the immobilized CAT with AC encapsulated by Gel as the supporter showed the highest relative enzymatic activity and a high stability in a broad range of pH values and temperatures, and its residual activity was 80% after 15 uses, whereas the immobilized CAT with AC as the supporter was retained at a level of only 50% under the same conditions. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 1498–1502, 2013

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