Development in the area of glycosylated membranes has been actively pursued in the past few years. This kind of promising biomimetic material is inspired by cell membranes. The recent surge of interest in these glycosylated membranes stems from their widespread number of applications to many areas in science and technology. With the glycosylation strategy, membrane separation properties, such as flux and antifouling, are greatly improved. Moreover, the ability to modulate biocompatibility, protein recognition, separation of biomolecules, enzyme immobilization, cell culture, and microorganisms capture are important in a variety of biological and medical applications. This review focuses on the recent progress in the preparation of these glycosylated membranes and highlights their applications. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39658.