UV-initiated copolymerization route for facile fabrication of epoxy-functionalized micro-zone plates
Article first published online: 9 AUG 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Applied Polymer Science
Volume 131, Issue 2, January 15, 2014
How to Cite
2014), UV-initiated copolymerization route for facile fabrication of epoxy-functionalized micro-zone plates. J. Appl. Polym. Sci., 131, doi: 10.1002/app.39787, , , (
- Issue published online: 23 OCT 2013
- Article first published online: 9 AUG 2013
- Manuscript Accepted: 22 JUL 2013
- Manuscript Received: 6 APR 2013
Bisphenol A-based epoxy acrylate (BABEA), a commercial ultraviolet (UV)-curable material, was introduced as a new manufacturing material for facile fabrication of epoxy-functionalized micro-zone plates through UV-initiated copolymerization using glycidyl methacrylate (GMA) as the functional monomer. The poly (BABEA-co-GMA) was highly transparent in visible range while highly opaque when the wavelength is less than 295 nm, and of high replication fidelity. X-ray photoelectron spectroscope (XPS) results indicated the existence of epoxy groups on the surface of the poly (BABEA-co-GMA), which allowed for binding protein through an epoxy-amino group reaction. A fabrication procedure was proposed for manufacturing BABEA based epoxy-functionalized micro-zone plates. The fabrication procedure was very simple; obviating the need of micromachining equipments, wet etching or imprinting techniques. To evaluate the BABEA-based epoxy-functionalized micro-zone plates, α-fetoprotein (AFP) was immobilized onto the capture zone for chemiluminescent (CL) detection in a noncompetitive immune response format. The proposed AFP immunoaffinity micro-zone plate was demonstrated as a low cost, flexible, homogeneous, and stable assay for α-fetoprotein (AFP). © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39787.