Get access

Preparation of bioactive glycosylated glial cell-line derived neurotrophic factor-loaded microspheres for medical applications

Authors

  • Saowapa Thumsing,

    1. The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Thailand
    Search for more papers by this author
  • Nipan Israsena,

    1. Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    2. The Stem Cell and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    3. The Neuroscience of Headache Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    Search for more papers by this author
  • Chatikorn Boonkrai,

    1. The Stem Cell and Cell Therapy Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    2. The Neuroscience of Headache Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
    Search for more papers by this author
  • Pitt Supaphol

    Corresponding author
    1. The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Thailand
    2. The Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University, Bangkok, Thailand
    Search for more papers by this author

ABSTRACT

Poly (lactic-co-glycolic acid) (PLGA)-coated gelatin microspheres containing glial cell-line derived neurotrophic factor (GDNF) were developed by thermal gelation through a water-in-oil emulsion technique. Gelatin types (A and B) at four different pH levels were investigated for their influences on the morphology, the microsphere size, the zeta potential, and the swelling ability. The encapsulation of GDNF and the release characteristics of GDNF were also determined using enzyme-linked immunosorbent assay (ELISA). The maximum cumulative released amounts of GDNF from the microspheres were increased from 50 to 90% after 4 d (based on the actual amount of the GDNF). Thus, the release of the GDNF contents in the microspheres depends on the amount of GDNF. Trigeminal ganglion cells (TGCs) were used to study the bioactivity of GDNF released from the microspheres, which was proven to retain its bioactivity in promoting the TGCs' neurite outgrowth. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40167.

Get access to the full text of this article

Ancillary