Enzymatic processes provide new perspectives for the modification of lignocellulose materials. Lignin is an excellent substrate for laccase, and the modification of lignin-rich jute fabric via graft polymerization with acrylamide (AAm) mediated by laccase and tert-butyl hydroperoxide (t-BHP) was investigated in this study. The products obtained were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. The grafting rate was determined in terms of elemental analysis. The hydrophilicity and thermal and dyeing properties of the modified jute fabric were studied. The results supported the conclusion that the polyacrylamide was grafted on the lignin of the jute fiber by laccase in coordination with t-BHP, representing a grafting rate of 2.87%. The hydrophilicity, thermostability, dye uptake, and dyeing depth of the jute fiber were increased after the enzymatic graft modification with AAm. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40387.