This study emphasizes microbial mediated transformation of sunflower oil to an adhesive product and characterization in detail. Marine bacterial isolates Bacillus (MTCC 5514), when grown in mineral medium, releases both hydrolytic enzymes and surface-active components during the log phase of growth. When this species was grown in the presence of sunflower oil at an optimized concentration of 5% (w/v) under room temperature, enzymatic hydrolysis of oil proceeds with the release of fatty acids and glycerol. Further, on increasing the incubation period, the presence of surface-active components, lipase and glycerol, influence the dimerization of the fatty acids, which further, transformed to a polymerized product sunflower oil-based adhesive product with adhesive nature. Liquid chromatography-mass spectrometry (electrospray ionization) analysis further authenticates the presence of dimeracids. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40555.