Vinyl acetate was grafted onto poly(ethylene terephthalate) (PET) fibers under electron-beam co-irradiation. Then, the grafted products were hydrolyzed under HCl solution to obtain PET-g-poly(vinyl alcohol) (PVA) fibers. Afterward, to impart superior hydrophilicity and skin care, collagen was immobilized onto PET-g-PVA fibers with glutaraldehyde. The modified fabric was characterized in terms of moisture regain, wettability, and evaporation rate [Ev (%/h)]. The degree of alcoholysis of the grafting product was more than 90% in a 10% HCl solution at 100°C for 4 h, and its moisture regain was 1.4%. The modified PET fabric with collagen showed a shorter drip diffusion time and a higher wicking height than the unmodified PET and PET-g-PVA fabric before and after multiple washings. The evaporation ratio and Ev of the collagen-immobilized PET fibers were far below those of the virgin PET fibers. The chemical structure, surface morphology, and element content of the product were characterized by attenuated total reflectance–Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. This further certified that the collagen-immobilized PET-g-PVA fiber was successfully prepared by the previous procedure. Furthermore, the PET–PVA–collagen fabric was capable of withstanding multiple washing cycles. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40597.