Preparation, characterization, and potential biomedical application of composite sponges based on collagen from silver carp skin
Article first published online: 14 JUN 2014
Copyright © 2014 Wiley Periodicals, Inc.
Journal of Applied Polymer Science
Volume 131, Issue 21, November 5, 2014
How to Cite
2014), Preparation, characterization, and potential biomedical application of composite sponges based on collagen from silver carp skin. J. Appl. Polym. Sci., 131, 40998, doi: 10.1002/app.40998, , , , , and (
- Issue published online: 11 AUG 2014
- Article first published online: 14 JUN 2014
- Manuscript Accepted: 6 MAY 2014
- Manuscript Received: 6 JAN 2014
- Scientific Major Research Project of Fujian Province . Grant Number: 2010NZ0001-1
- Natural Science Foundation of Fujian Province . Grant Numbers: 2012J01209 , 2013J01388
- Key Laboratory of Biomedical Materials of Tianjin
- silver carp skin
To reuse fish processing waste for biomedical materials, collagen (Col) was extracted from silver carp skin, and Col–chitosan (Ch) composite sponges were prepared by a freeze-drying method. The atomic force microscopy and electrophoresis results suggest the Col might have been type I. To obtain the optimum conditions for the manufacture of the Col–Ch sponges, the characteristics of sponges composed of different ratios of Col to Ch with different crosslinkers were evaluated. Scanning electron microscopy showed that the sponges had an interconnected network structure with porosity. Infrared spectroscopy demonstrated that intermolecular crosslinkages between Col and Ch occurred. The swelling measurements implied that all of the sponges could bind an 18- to 36-fold amount of distilled water and still maintain their form and stability. When the ratio of Col to Ch was higher than 1:0.25, the swelling and degradation rate decreased with increasing Ch. Cell proliferation, hemolysis, and hemostasis assay indicated that the sponges exhibited noncytotoxicity, biocompatibility, nonhemolysis, and hemostatic efficacy. Overall, we concluded that the optimal ratio of Col and Ch for the sponges was 1:0.25, and glutaraldehyde crosslinking was more suitable than 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. These results demonstrate the potential application of silver carp skin Col–Ch sponges for tissue engineering and wound dressing in non-weight-bearing tissue. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40998.