Preparation and release characteristics of dexamethasone acetate loaded organochlorine-free poly(lactide-co-glycolide) nanoparticles

Authors

  • Izabella Maria Ferreira Campos,

    1. Department of Chemical Engineering, School of Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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  • Thiago Melo Santos,

    1. Department of Chemical Engineering, School of Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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  • Gabriella Maria Fernandes Cunha,

    1. Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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  • Kláudia Maria Machado Neves Silva,

    1. Department of Chemical Engineering, School of Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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  • Rosana Zacarias Domingues,

    1. Department of Chemistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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  • Armando da Silva Cunha Júnior,

    1. Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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  • Kátia Cecília de Souza Figueiredo

    Corresponding author
    1. Department of Chemical Engineering, School of Engineering, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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ABSTRACT

Dexamethasone-loaded poly(lactide-co-glycolide) (PLGA) devices are commonly used as model systems for controlled release. In this study, PLGA nanoparticles containing dexamethasone acetate were prepared by a nanoprecipitation technique in the absence of organochlorine solvents and were characterized by their mean size, ζ potential, scanning electron microscopy, and differential scanning calorimetry to develop a controlled release system. The analytical method for the quantification of dexamethasone acetate by high-performance liquid chromatography was validated. The results show that it was possible to prepare particles at a nanometric size because the average diameter of the drug-loaded PLGA particles was 540 ± 4 nm with a polydispersity index of 0.07 ± 0.01 and a ζ potential of −2.5 ± 0.3 mV. These values remained stable for at least 7 months. The drug encapsulation efficiency was 48%. In vitro tests showed that about 25% of the drug was released in 48 h. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 41199.

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