A composite scaffold of gelatine (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was successfully fabricated. Growth factors such as bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were loaded into the Gel-Pec-BCP hydrogel scaffolds by freeze-drying. The surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive and X-ray diffraction spectroscopy. The results obtained from Fourier transform infrared spectroscopy and quantitative measurements showed successfully loading of BMP-2 and VEGF into the Gel-Pec-BCP hydrogel scaffolds. In addition MC3T3-E1 preosteoblasts were cultivated on the three types of scaffolds to investigate the effects of BMP-2 and VEGF on cell viability and proliferation. The Gel-Pec-BCP scaffolds loaded with VEGF and BMP-2 demonstrated more cell spreading and proliferation compared to those of the Gel-Pec-BCP scaffolds. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41241.