Cell sheet engineering for regenerative medicine provides an alternative to the use of biodegradable scaffold materials and the injection of single cell suspensions. A novel temperature-responsive tissue culture surface is prepared by Sopita Wong-In et al. on page 3061 using ultraviolet irradiation to graft poly(N-isopropylacrylamide-co-acrylamide) onto commercial tissue culture surfaces. The cells grow as successfully on the grafted surface as those cultured on the ungrafted surface. When the temperature is reduced, the confluent cells can be detached as a continuous sheet that preserves tight junctions and extracellular matrix proteins, allowing the creation of multilayered sheets. Cell morphology, shown in the cover image, is photographed under a bright-field inverted microscope.