• benthos;
  • endangered species;
  • freshwater mussels;
  • invertebrates;
  • nutrient enrichment;
  • river;
  • stream;
  • water quality


  • 1.
    Studies from Central Europe have shown a relationship between the impaired population status of threatened freshwater mussel species and elevated nitrate nitrogen (N−NOmath image) concentrations in running waters.
  • 2.
    Causal mechanisms, however, remain unknown, and no experimental data or comprehensive studies involving more species are available, which causes uncertainty in prioritizing conservation actions.
  • 3.
    This study uses both descriptive and experimental approaches to identify the effects of nitrates on freshwater mussels and demonstrates the need for integrating different research methods for development of conservation strategies for threatened species.
  • 4.
    Spatial co-occurrence of five native freshwater mussel species (Anodonta anatina, Pseudanodonta complanata, Unio pictorum, Unio tumidus, Unio crassus) and N−NOmath image concentrations were examined in a 7th-order river catchment (Lužnice River, Czech Republic) with anthropogenically-induced increasing N−NOmath image levels and declining populations of these species during the 20th century.
  • 5.
    Acute toxicity of N−NOmath image was then estimated for artificially reared juveniles of A. anatina and U. crassus using both lethal and sublethal test endpoints.
  • 6.
    Results showed that the probability of occurrence of all species was significantly reduced in reaches with elevated N−NOmath image levels.
  • 7.
    In contrast, the results of toxicity testing revealed that the juvenile stages of the two tested species were less sensitive to N−NOmath image than most previously tested freshwater macroinvertebrates. The detected 96-h median lethal N−NOmath image concentrations were two orders of magnitude higher than the limits derived from distributional data.
  • 8.
    Despite the probable absence of a direct negative effect of N−NOmath image on freshwater mussel populations, N−NOmath image has potential to be used as an effective indicator of biotope conditions. Identification of causal mechanisms responsible for the observed relationship between unionids and N−NOmath image will require further research. Copyright © 2009 John Wiley & Sons, Ltd.